Genotyping of red tilapia strains in Central Luzon Philippines targeting ESR1 gene
##plugins.themes.bootstrap3.article.sidebar##
Published
Jun 10, 2024
##plugins.themes.bootstrap3.article.main##
Arren Christian M. de Guia
Khristina Judan Cruz
Karl Danielle D. Almuete
Mary Rose D. Uy
Abstract
Tilapia is an economically important commodity worldwide. Marker-assisted selection (MAS) has been practiced in livestock culture to ensure the selection of a specific trait that will benefit the culture system. This study assessed the allelic variation in six red tilapia strains in Central Luzon, Philippines. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was done to evaluate the genotypes of six strains of red tilapia using intron one portion of the chromosome one of the estrogen receptor 1 (ESR1) gene. The target gene was amplified using primers designed in previous study and then subjected to RFLP using PvuII restriction enzyme. Single Nucleotide Polymorphisms (SNPs) were also evaluated in six DNA sequences. Results showed that 18 red tilapia samples were found to be of the AA genotype. Aligned sequences of the 122 bp ESR1 gene revealed that among six red tilapia strains, there are 117 identical pairs, three transversional pairs, and three transitional pairs. The SNPs observed can be used to identify restriction enzymes that discriminate different genotypes in the target gene. Association studies can be done to determine the genotypes that are linked to specific traits in cultured animals.
How to Cite
Guia ACM de, Cruz KJ, Almuete KDD, Uy MRD. 2024. Genotyping of red tilapia strains in Central Luzon Philippines targeting ESR1 gene. The Palawan Scientist. 16(1):8–14. https://doi.org/10.69721/TPS.J.2024.16.1.02.
Downloads
Download data is not yet available.
##plugins.themes.bootstrap3.article.details##
References
Balcazar JL, Aguirre A, Gómez G and Paredes W. 2004. Culture of hybrid Red Tilapia (Oreochromis mossambicus X Oreochromis niloticus) in marine cages: Effects of stocking density on survival and growth. Agricultural and Food Sciences, 14(2): 10-12.
Belton B, Little D and Young J. 2006. Red tilapia cage culture in central Thailand. Aquaculture Asia Magazine, 11(3): 28-30.
DOF (Department of Fisheries). 2005. Freshwater Fish Farm Production, 2003. Bangkok, Thailand. https://library.enaca.org/AquacultureAsia/Articles/July-Sept-2006/July-Sept-2006.pdf. Accessed on 07 March 2022.
Eknath AE, Bentsen HB, Gjerde B, Tayamen MM, Abella TA, Gjedrem T and Pullin RSVP. 1991. Approaches to national fish breeding programs: Pointers from a tilapia pilot study. NAGA 14(2): 10-12. https://hdl.handle.net/20.500.12348/3116
Hamzah A, Nguyen NH, Yee HY and Ponzoni RW. 2009. A complete diallel cross involving three strains of red tilapia reared in earthen pond. Asian Pacific Aquaculture Conference. pp. 4-19. https://research.usc.edu.a u/esploro/outputs/99450488302621. Accessed on 07 March 2022.
Galman OR, Moreau J and Avtalion RR. 1988. Breeding characteristics and growth performance of red tilapia. The Second International Symposium on Tilapia in Aquaculture, ICLARM Conference Proceedings, 15:169-175.
Gupta MV and Acosta BO. 2004. A review of global tilapia farming practices. Aquaculture Asia Magazine, 9: 7-12.
McAndrew BJ, Roubal FR, Roberts RJ, Bullock AM and McEwen IM. 1988. The genetics and histology of red, blond and associated colour variants in Oreochromis niloticus. Genetica, 76: 127-137. https://doi.org/10.1007/BF00058811
Popma T and Masser M. 1999. Tilapia; Life History and Biology. Southern Regional Aquaculture Center, Auburn, USA, 283pp.
Rothschild MF, Larson RG, Jacobson C and Pearson P. 1991. PvuII polymorphisms at the porcine oestrogen receptor locus (ESR). Animal Genetics, 22(5): 448. https://doi.org/10.1111/j.1365-2052.1991.tb00715.x
Short TH, Rothschild MF, Southwood OI, McLaren DG, de Vries A, van der Steen H, Eckardt GR, Tuggle CK, Helm J, Vaske DA et al. 1997. Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines. Journal of Animal Science, 75(12): 3138–3142. https://doi.org/10.2527/1997.75123138x
Soller M and Beckmann, LS. 1983. Genetic polymorphism in varietal identification and crop improvement. Theoretical and Applied Genetics, 67: 25-33. https://doi.org/10.1007/bf00303917
Vadhel NP, Akolkar NG, Pathan J, Tandel JT, Lende S and Shrivastava V. 2016. Red Tilapia: A candidate euryhaline species for aqua farming in Gujarat. Journal of Fisheries Sciences, 11(1): 48-50. http://dx.doi.org/10.21767/1307-234X.1000107
Watanabe WO, Ellingson LJ and Olla BL. 1990. Salinity tolerance and seawater survival vary ontogenetically in Florida red tilapia. Aquaculture, 87: 311–321. https://doi.org/10.1016/0044-8486(90)90068-X
Wu Y, Pan AL, Pi JS, Pu YJ, Du JP, Liang ZH and Shen J. 2015. SNP analysis reveals estrogen receptor 1 (ESR1) gene variants associated with laying traits in quails. Archives Animal Breeding, 58: 441–444. https://doi.org/10.5194/aab-58-441-2015
Vicencio NG, Viernes VD, Ocampo LC and Ocampo MB. 2017. Gross anatomy of the female reproductive organs of PNP (Sus scrofa L.). International Journal of Agricultural Technology, 13(7.2): 1627-1638.
Yan L, Feng H, Wang F, Lu B, Liu X, Sun L and Wang D. 2019. Establishment of three estrogen receptors (esr1, esr2a, esr2b) knockout lines for functional study in Nile tilapia. Journal of Steroid Biochemistry and Molecular Biology, 191: 105379. https://doi.org/10.1016/j.jsbmb.2019.105379
Belton B, Little D and Young J. 2006. Red tilapia cage culture in central Thailand. Aquaculture Asia Magazine, 11(3): 28-30.
DOF (Department of Fisheries). 2005. Freshwater Fish Farm Production, 2003. Bangkok, Thailand. https://library.enaca.org/AquacultureAsia/Articles/July-Sept-2006/July-Sept-2006.pdf. Accessed on 07 March 2022.
Eknath AE, Bentsen HB, Gjerde B, Tayamen MM, Abella TA, Gjedrem T and Pullin RSVP. 1991. Approaches to national fish breeding programs: Pointers from a tilapia pilot study. NAGA 14(2): 10-12. https://hdl.handle.net/20.500.12348/3116
Hamzah A, Nguyen NH, Yee HY and Ponzoni RW. 2009. A complete diallel cross involving three strains of red tilapia reared in earthen pond. Asian Pacific Aquaculture Conference. pp. 4-19. https://research.usc.edu.a u/esploro/outputs/99450488302621. Accessed on 07 March 2022.
Galman OR, Moreau J and Avtalion RR. 1988. Breeding characteristics and growth performance of red tilapia. The Second International Symposium on Tilapia in Aquaculture, ICLARM Conference Proceedings, 15:169-175.
Gupta MV and Acosta BO. 2004. A review of global tilapia farming practices. Aquaculture Asia Magazine, 9: 7-12.
McAndrew BJ, Roubal FR, Roberts RJ, Bullock AM and McEwen IM. 1988. The genetics and histology of red, blond and associated colour variants in Oreochromis niloticus. Genetica, 76: 127-137. https://doi.org/10.1007/BF00058811
Popma T and Masser M. 1999. Tilapia; Life History and Biology. Southern Regional Aquaculture Center, Auburn, USA, 283pp.
Rothschild MF, Larson RG, Jacobson C and Pearson P. 1991. PvuII polymorphisms at the porcine oestrogen receptor locus (ESR). Animal Genetics, 22(5): 448. https://doi.org/10.1111/j.1365-2052.1991.tb00715.x
Short TH, Rothschild MF, Southwood OI, McLaren DG, de Vries A, van der Steen H, Eckardt GR, Tuggle CK, Helm J, Vaske DA et al. 1997. Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines. Journal of Animal Science, 75(12): 3138–3142. https://doi.org/10.2527/1997.75123138x
Soller M and Beckmann, LS. 1983. Genetic polymorphism in varietal identification and crop improvement. Theoretical and Applied Genetics, 67: 25-33. https://doi.org/10.1007/bf00303917
Vadhel NP, Akolkar NG, Pathan J, Tandel JT, Lende S and Shrivastava V. 2016. Red Tilapia: A candidate euryhaline species for aqua farming in Gujarat. Journal of Fisheries Sciences, 11(1): 48-50. http://dx.doi.org/10.21767/1307-234X.1000107
Watanabe WO, Ellingson LJ and Olla BL. 1990. Salinity tolerance and seawater survival vary ontogenetically in Florida red tilapia. Aquaculture, 87: 311–321. https://doi.org/10.1016/0044-8486(90)90068-X
Wu Y, Pan AL, Pi JS, Pu YJ, Du JP, Liang ZH and Shen J. 2015. SNP analysis reveals estrogen receptor 1 (ESR1) gene variants associated with laying traits in quails. Archives Animal Breeding, 58: 441–444. https://doi.org/10.5194/aab-58-441-2015
Vicencio NG, Viernes VD, Ocampo LC and Ocampo MB. 2017. Gross anatomy of the female reproductive organs of PNP (Sus scrofa L.). International Journal of Agricultural Technology, 13(7.2): 1627-1638.
Yan L, Feng H, Wang F, Lu B, Liu X, Sun L and Wang D. 2019. Establishment of three estrogen receptors (esr1, esr2a, esr2b) knockout lines for functional study in Nile tilapia. Journal of Steroid Biochemistry and Molecular Biology, 191: 105379. https://doi.org/10.1016/j.jsbmb.2019.105379
Section
Original Article
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.